Application
Western blot analysis of human recombinant Mucin 5B fusion protein.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic and cell surface localization.| Product Name | Mucin 5B Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1201-1500 mapping near the C-terminus of Mucin 5B of human origin |
| Modification Notes | Mucins are a group of high molecular weight glycoproteins consisting of a mucin core protein and O-linked carbohydrates. The Mucin 5B gene, which contains a 3’ cis-element, is one of the four mucin genes mapped to human chromosome 11p15. Although Mucin 5B is the prominent human gallbladder mucin, it is also expressed and secreted in the colon. In addition, Mucin 5B is expressed in non-inflammed middle ears and normal esophagus, and is upregulated by chronic inflammation and highly secreted in the diseased middle ear. Mucin 5B is abnormally expressed in gastric carcinomatous tissues. Its expression in gastric cancer cells is controlled by a highly active distal promoter, which is upregulated by protein kinase C and repressed under the influence of methylation. Mucous differentiation and expression of Mucin 5B is retinoic acid- (RA) or retinol-dependent. RA control of mucin gene is mediated by the retinoid acid receptor RARa and, to a lesser extent, by RARg. The correlation of mucin protein levels in human cervical mucous with the peak at midcycle suggests that mucin may be important in sperm transit to the uterus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Mucin 5B of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1201-1500 mapping near the C-terminus of Mucin 5B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1201-1500 mapping near the C-terminus of Mucin 5B of human origin |
| Entrez Gene | 727897 |
|---|
Application
Western blot analysis of human recombinant Mucin 5B fusion protein.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic and cell surface localization.| Application Notes | recommended for detection of Mucin 5B of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
