Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing membrane (cell-cell adhesion) staining of myocytes.Application
Western blot analysis of N-cadherin expression in mouse brain tissue extract.Application
Western blot analysis of N-cadherin expression in non-transfected: (A) and mouse N-cadherin transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane (cell-cell adhesion) staining.Application
Western blot analysis of N-cadherin expression in PC-12 whole cell lysate.| Product Name | N-cadherin Antibody (H-63) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The most distal of these cadherins is thought to be responsible for binding specificity, transmembrane domains and carboxy-terminal intracellular domains. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as b-catenin, to regulate cadherin function. Members of this family of adhesion proteins include rat cadherin K (and its human homolog, cadherin-6), R-cadherin, B-cadherin, E/P cadherin and cadherin-5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of N-cadherin of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 450-512 mapping within the extracellular domain of N-cadherin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 450-512 mapping within the extracellular domain of N-cadherin of human origin |
| Entrez Gene | 1000 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing membrane (cell-cell adhesion) staining of myocytes.
Application
Western blot analysis of N-cadherin expression in mouse brain tissue extract.
Application
Western blot analysis of N-cadherin expression in non-transfected: (A) and mouse N-cadherin transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane (cell-cell adhesion) staining.
Application
Western blot analysis of N-cadherin expression in PC-12 whole cell lysate.| Application Notes | recommended for detection of N-cadherin of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
