Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomerulus and tubules.Application
Western blot analysis of TMPRSS2 expression in mouse prostate tissue extract.Application
Western blot analysis of TMPRSS2 expression in HeLa whole cell lysate.Application
Western blot analysis of TMPRSS2 expression in non-transfected: (A) and mouse TMPRSS2 transfected: (B) 293T whole cell lysates.| Product Name | TMPRSS2 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin |
| Modification Notes | Extracellular proteases mediate the digestion of neighboring extracellular matrix components in initial tumor growth, allow desquamation of tumor cells into the surrounding environment, provide the basis for invasion of basement membranes in targeted metastatic organs and are required for release and activation of many growth and angiogenic factors (1,2). The TMPRSS2 gene encodes a 492 amino acid multimeric serine protease, which is mainly expressed in the mouse prostate and kidney, and is also expressed in the human small intestine, prostate, colon, stomach and salivary gland (3,4). TMPRSS2 contains several domains, including a serine protease domain of the S1 family, a scavenger receptor cysteine-rich domain of group A, an LDL receptor class A domain and a transmembrane domain (3). TMPRSS2 is expressed as a full length form and a cleaved protease domain (5) and its expression is increased by androgenic hormones. TMPRSS2 is also expressed in prostate carcinoma, suggesting that it may play a role in prostate carcinogenesis (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TMPRSS2 catalytic chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 296-345 mapping within an internal region of TMPRSS2 of human origin |
| Entrez Gene | 7113 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomerulus and tubules.
Application
Western blot analysis of TMPRSS2 expression in mouse prostate tissue extract.
Application
Western blot analysis of TMPRSS2 expression in HeLa whole cell lysate.
Application
Western blot analysis of TMPRSS2 expression in non-transfected: (A) and mouse TMPRSS2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TMPRSS2 catalytic chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
