Application
Western blot analysis of Y14 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing nuclear localization.| Product Name | Y14 Antibody (Q-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Y14 of human origin and RBM8 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20–24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is colocalized with oskar mRNA at the posterior pole of the cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Y14 of human origin and RBM8 of mouse and rat origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of Y14 of human origin and RBM8 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 9939 |
|---|
Application
Western blot analysis of Y14 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human heart tissue showing nuclear localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
