Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of α-Adaptin 1/2 expression in KNRK (A) and Hep G2 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic staining.| Product Name | α-Adaptin 1/2 Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 678-977 (deletion at 707-728) of α-Adaptin 2 of mouse origin |
| Modification Notes | Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of clathrin binding adaptin proteins (©-Adaptin and ∫-Adaptin for AP-1; å-Adaptin 1, å-Adaptin 2 and ∫2-Adaptin for AP-2) and two smaller subunits known as AP50 and AP17. The å- and ∫- Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. å-Adaptin splice variants 1 and 2 display variable relative expression levels and differential distribution in different tissues. AP180 (also designated AP-3 or F1-20) is a synapse-specific clathrin assembly protein. The protein CALM (clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in clathrin assembly. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of α-Adaptin 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 678-977 (deletion at 707-728) of &alpha -Adaptin 2 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 678-977 (deletion at 707-728) of α-Adaptin 2 of mouse origin |
| Entrez Gene | 161 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of α-Adaptin 1/2 expression in KNRK (A) and Hep G2 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic staining.| Application Notes | recommended for detection of α-Adaptin 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
