Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp.Application
Western blot analysis of CD8-α expression in non-transfected: (A) and human CD8-α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD8-α expression in non-transfected: (A) and human CD8-α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD8-α expression in human PBL whole cell lysate.Application
Western blot analysis of CD8-α expression in mouse PBL whole cell lysate.Application
Western blot analysis of human recombinant CD8-α fusion protein.| Product Name | CD8-α Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 22-182 representing the extracellular domain of CD8 alpha chain of human origin |
| Modification Notes | The T cell receptor (TCR) is a heterodimer composed of either å and ∫ or © and ∂ chains. CD3 chains and the CD4 or CD8 (CD8-å and CD8-∫) co-receptors are also required for efficient signal transduction through the TCR. The TCR is expressed on T helper and T cytotoxic cells that can be distinguished by their expression of CD4 and CD8 proteins; T helper cells express CD4 proteins and T cytotoxic cells display CD8 proteins. CD8s are cell surface glycoproteins that exist as two chain complex (åå or å∫) receptors that bind class I MHC molecules presented by the antigen-presenting cell (APC). A primary function of CD8 proteins is to facilitate antigen recognition by the TCR and to strengthen the avidity of the TCR-antigen interactions. An additional role for CD8-expressing T cells may be to maintain low levels of HIV expression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD8 alpha chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 22-182 representing the extracellular domain of CD8 alpha chain of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 22-182 representing the extracellular domain of CD8 alpha chain of human origin |
| Entrez Gene | 925 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing cytoplasmic staining of cells in white pulp.
Application
Western blot analysis of CD8-α expression in non-transfected: (A) and human CD8-α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD8-α expression in non-transfected: (A) and human CD8-α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD8-α expression in human PBL whole cell lysate.
Application
Western blot analysis of CD8-α expression in mouse PBL whole cell lysate.
Application
Western blot analysis of human recombinant CD8-α fusion protein.| Application Notes | recommended for detection of CD8 alpha chain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
