Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.| Product Name | CatSper Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | CatSper is an ion transport protein located on the surface of sperm cells. CatSper (for cation channel of sperm) is vital to cAMP-mediated calcium influx in sperm, sperm motility and fertilization. The gene that encodes CatSper maps to human chromosome 11q12.1. The 686 amino acid protein is a single, six-transmembrane-spanning protein of the voltage- dependent Ca2+ channel four-repeat structure and is expressed exclusively in the testis. In CatSper -/- sperm, neither cAMP nor cGMP elicit a significant Ca2+ influx in both mutant and wild-type sperm. CatSper is localized to the sperm flagellum and CatSper -/- mice show sluggish and less directed movements. CatSper is required to penetrate the egg, but is not required for egg activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CatSper of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 481-780 mapping at the C-terminus of CatSper of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 481-780 mapping at the C-terminus of CatSper of human origin |
| Entrez Gene | 117144 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.| Application Notes | recommended for detection of CatSper of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
