Application
Western blot analysis of Aldolase B expression in non-transfected: (A) and mouse Aldolase B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Aldolase B expression in non-transfected: (A) and mouse Aldolase B transfected: (B) 293T whole cell lysates.| Product Name | Aldolase B Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate (1). Fructose 1,6-bisphosphate aldolase exists as three forms, the muscle-specific Aldolase A, the liver-specific aldolase B, and the brain-specific aldolase C (2). Aldolase A, B, and C arose from a common ancestral gene, from which aldolase B first diverged (3). Aldolase A is one of the most highly conserved enzymes known, with only about 2% of the residues changing per 100 million years (4). Aldolase B is regulated by the hormones insulin and glucagon and has been implicated in hereditary fructose intolerance disease (3,5). Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells (6,8-10). Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells (7,10). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Aldolase B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 315-364 mapping at the C-terminus of Aldolase B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 315-364 mapping at the C-terminus of Aldolase B of human origin |
| Entrez Gene | 229 |
|---|
Application
Western blot analysis of Aldolase B expression in non-transfected: (A) and mouse Aldolase B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Aldolase B expression in non-transfected: (A) and mouse Aldolase B transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Aldolase B of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
