Application
Western blot analysis of calsequestrin 2 expression in non-transfected: (A) and human calsequestrin 2 transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).Application
Western blot analysis of human recombinant calsequestrin 2 fusion protein.| Product Name | calsequestrin 2 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Calsequestrin (CS), also known as CSQ, is the major calcium-binding protein of cardiac and skeletal muscle whose function is to sequester calcium in the lumen of the sarcoplasmic reticulum (SR). In mammals, there are two forms of this protein, calsequestrin 1 and calsequestrin 2, which encode fast-twitch skeletal muscle and cardiac calsequestrin, respectively. The form of calsequestrin 1 known as Calmitin is located in the terminal cisternae luminal spaces of the SR of fast skeletal muscle cells. Calsequestrin 2 is present in the terminal cisternae luminal spaces of the SR in both cardiac and slow skeletal muscle cells. In addition, calsequestrin regulates the ryanodine receptor signalling (RyR) through Triadin and Junctin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of calsequestrin 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-80 mapping near the N-terminus of calsequestrin 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-80 mapping near the N-terminus of calsequestrin 2 of human origin |
| Entrez Gene | 845 |
|---|
Application
Western blot analysis of calsequestrin 2 expression in non-transfected: (A) and human calsequestrin 2 transfected: (B) 293T whole cell lysates and mouse heart tissue extract (C).
Application
Western blot analysis of human recombinant calsequestrin 2 fusion protein.| Application Notes | recommended for detection of calsequestrin 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
