Application
Western blot analysis of ZO-2 expression in non-transfected: (A) and mouse ZO-2 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing membrane and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of ZO-2 expression in non-transfected control (A) and ZO-2 siRNA transfected (B) HeLa cells. Blot probed with ZO-2 (H-110): . α-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of ZO-2 expression in HeLa whole cell lysate.| Product Name | ZO-2 Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Tight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane-associated guanylate kinase (MAGUK) family and include occludin, ZO-1, ZO-2 and ZO-3 (1,2). These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain (3). ZO-1 forms complexes with either ZO-2 or ZO-3 (4). In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction (5-7). ZO-1 expression is significantly reduced in many breast cancer lines (8). ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue (2,3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ZO-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 391-500 mapping to an internal region of ZO-2 (Zonula occludens-2) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 391-500 mapping to an internal region of ZO-2 (Zonula occludens-2) of human origin |
| Entrez Gene | 9414 |
|---|
Application
Western blot analysis of ZO-2 expression in non-transfected: (A) and mouse ZO-2 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colo-rectal cancer tissue showing membrane and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of ZO-2 expression in non-transfected control (A) and ZO-2 siRNA transfected (B) HeLa cells. Blot probed with ZO-2 (H-110): . α-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of ZO-2 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of ZO-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
