Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of IDO expression in non-transfected: (A) and human IDO transfected: (B) 293T whole cell lysates.| Product Name | IDO Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Indoleamine 2,3-dioxygenase (IDO) is an IFN-© inducible enzyme that catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. The gene encoding human IDO maps to chromosome 8p12-p11. IDO, also known as INDO, is an important modulator of immunological responses and protects allogeneic concepti from alloreactive maternal lymphocytes. IDO mediates an interesting inhibitory effect of HeLa cells co-cultured with human PBLs. The ILN-2-induced proliferation response of PBLs is diminished in the presence of HeLa cells while an IDO inhbitor negates this effect. Flow cytometric analysis indicates both mature and immature CD123-positive dentritic cells suppress T cell activity using IDO. IDO-transfected cells co-cultured with T cells reduces T cell proliferation. Additionally, adopted transfer of donor T cells reduces donor T cell numbers in IDO-transgenic mice. The pharmacological or genetic manipulation of IDO may be useful for supressing undesirable T cell response. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IDO of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 271-380 of IDO of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 271-380 of IDO of human origin |
| Entrez Gene | 3620 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of IDO expression in non-transfected: (A) and human IDO transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IDO of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
