Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells and neuropil.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of EAAT1 expression in EOC 20 whole cell lysate.Application
Western blot analysis of EAAT1 expression in non-transfected control (A) and EAAT1 siRNA transfected (B) HeLa cells. Blot probed with EAAT1 (H-50): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of EAAT1 expression in rat cerebellum tissue extract.| Product Name | EAAT1 Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Excitatory amino acid transporter 1 (EAAT1) is one of the two glial glutamate transporters that clear the extracellular glutamate generated during neuronal signal transmission. Excitatory amino acid transporters (EAATs) are membrane-bound proteins that are localized in glial cells and pre-synaptic glutamatergic nerve endings. EAATs transport the excitatory neurotransmitters L-glutamate and D-aspartate, a process that is essential for terminating the postsynaptic acction of glutamate. The reuptake of amino acid neurotransmitters by EAAT proteins has been shown to protect neurons from excitotoxicity, which is caused by the accumulation of amino acid neurotransmitters. Three glutamate transporters have been identified in human brain, designated EAAT1-3. EAAT1 and EAAT3 are also expressed in various non-nervous tissues, while EAAT2 expression appears to be restricted to the brain. Surface expression of the glial glutamate transporter EAAT1 is stimulated by insulin-like growth factor 1 through activation of phosphatidylinositol-3-kinase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of EAAT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-50 mapping at the N-terminus of EAAT1 (excitatory amino acid transporter 1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-50 mapping at the N-terminus of EAAT1 (excitatory amino acid transporter 1) of human origin |
| Entrez Gene | 6507 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells and neuropil.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of EAAT1 expression in EOC 20 whole cell lysate.
Application
Western blot analysis of EAAT1 expression in non-transfected control (A) and EAAT1 siRNA transfected (B) HeLa cells. Blot probed with EAAT1 (H-50): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of EAAT1 expression in rat cerebellum tissue extract.| Application Notes | recommended for detection of EAAT1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
