Application
Western blot analysis of connexin 40 expression in CCD-1064Sk (A) and Hs68 (B) whole cell lysates.| Product Name | connexin 40 Antibody (H-116) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The connexin family of proteins form hexameric complexes called “connexons” that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of 4 transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop, and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20 connexin isoforms produces channels with distinct permeabilities and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another. Consequently, a wide variety of malignant phenotypes associate with decreased connexin expression and gap junction communication, dependent on the particular connexin that is effected. For example, upregulation of connexin 40 following cardiac surgery can mark a susceptibility to post-operative atrial fibrillation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of connexin 40 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 231-346 mapping within a C-terminal cytoplasmic domain of connexin 40 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 231-346 mapping within a C-terminal cytoplasmic domain of connexin 40 of human origin |
| Entrez Gene | 2702 |
|---|
Application
Western blot analysis of connexin 40 expression in CCD-1064Sk (A) and Hs68 (B) whole cell lysates.| Application Notes | recommended for detection of connexin 40 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
