Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Product Name | RGS9 Antibody (H-231) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to various intracellular effectors. G proteins comprise å, ß and g subunits, and following activation the a subunit binds GTP and dissociates from the ßg complex (1). A large group of proteins have been identified as GTPase-activating proteins (GAPs), including the RGS (regulator of G protein signaling) family, which serve to deactivate specific Gå isoforms by increasing the rate at which they convert GTP to GDP (2,3). A subfamily of RGS proteins expressed in the central nervous system contain, in addition to the highly conserved RGS domain, a characteristic GGL domain, or G protein g subunit-like domain, which mediates binding to Gß 5 subunits (4,5). This subfamily, which includes RGS6, RGS7, RGS9 and RGS11, associates with Gß 5 to form active GAP complexes that are predominantly localized to the cytosol (6). RGS/ß5 complexes preferentially target Gåo subunit for hydrolysis and inhibit Gß1g2-mediated activation of phospholipase C (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RGS9 of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 444-674 of RGS9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 444-674 of RGS9 of human origin |
| Entrez Gene | 8787 |
|---|
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Application Notes | recommended for detection of RGS9 of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
