Application
Western blot analysis of HPK1 expression in non-transfected 293T: (A), mouse HPK1 transfected 293T: (B) and BJAB (C) whole cell lysates.Application
Western blot analysis of HPK1 expression in Ramos whole cell lysate.| Product Name | HPK1 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Several mammalian kinases have been identified with sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include HPK1, KHS, GLK, NIK, YSK1, Krs-1, Krs-2 and GC kinase. HPK1 (hematopoietic progenitor kinase 1), like many other STE20-like kinases, specifically activates the JNK signaling pathway. HPK1 binds to and phosphorylates MEKK, suggesting it plays an important role in regulating the stress responsive JNK/SAPK signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HPK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 291-490 of HPK1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 291-490 of HPK1 of human origin |
| Entrez Gene | 11184 |
|---|
Application
Western blot analysis of HPK1 expression in non-transfected 293T: (A), mouse HPK1 transfected 293T: (B) and BJAB (C) whole cell lysates.
Application
Western blot analysis of HPK1 expression in Ramos whole cell lysate.| Application Notes | recommended for detection of HPK1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
