Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic and nuclear localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts.Application
Western blot analysis of SMN expression in 293T whole cell lysate.Application
Western blot analysis of SMN expression in non-transfected: (A) and human SMN transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tumor tissue showing nuclear and cytoplasmic localization.Application
Western blot analysis of SMN expression in HeLa (A), SK-BR-3 (B) and K-562 nuclear extracts.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.| Product Name | SMN Antibody (H-195) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of motor neurons in the spinal cord. SMA is caused by deletion or loss-of-function mutations of SMN (survival of motor neuron) gene. SMN, also known as Gemin1, SMN1, SMNT and BCD541, exists as four isoforms produced by alternative splicing. SMN is oligomeric and forms a complex with Gemin2 (formerly SIP1), Gemin3 (a DEAD box RNA helicase), Gemin4, Gemin5 and Gemin6, as well as several spliceosomal snRNP proteins. The SMN complex plays an essential role in splicesomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing of the nucleus. The SMN complex is found in both the cytoplasm and the nucleus. The nuclear form is concentrated in subnuclear bodies called gems (gemini of the coiled bodies). Cytoplasmic SMN interacts with spliceosomal Sm proteins and facilitates their assembly onto U snRNAs, and nuclear SMN mediates recycling of pre-mRNA splicing factors. Nearly identical telomeric and centromeric forms of SMN encode the same protein; however, only mutations in the telomeric form are associated with the disease-state SMA. SMN is expresed in a wide variety of tissues including brain, kidney, liver, spinal cord and moderately in skeletal and cardiac muscle. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SMN of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-195 mapping at the N-terminus of SMN (survival of motor neurons) (also designated Gemin1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-195 mapping at the N-terminus of SMN (survival of motor neurons) (also designated Gemin1) of human origin |
| Entrez Gene | 6606 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic and nuclear localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts.
Application
Western blot analysis of SMN expression in 293T whole cell lysate.
Application
Western blot analysis of SMN expression in non-transfected: (A) and human SMN transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tumor tissue showing nuclear and cytoplasmic localization.
Application
Western blot analysis of SMN expression in HeLa (A), SK-BR-3 (B) and K-562 nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear staining.| Application Notes | recommended for detection of SMN of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
