Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Gα o expression in rat (A) and bovine (B) brain extracts.| Product Name | Gα o Antibody (K-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i.e., adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their å subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gå subunits have been identified; these include Gs, Gi, Gq and Ga 12/13. The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Gå i-1, Gå i-2, Gå i-3, Gå o, Gå t1, Gå t2, Gå z and Gå gust. Of these, the three Ga i subtypes function to open atrial potassium channels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of G&alpha o of mouse, rat, human and bovine origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a highly divergent domain of G&alpha o of rat origin |
|---|---|
| Tissue Specificity | epitope mapping within a highly divergent domain of Gα o of rat origin |
| Entrez Gene | 2775 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Gα o expression in rat (A) and bovine (B) brain extracts.| Application Notes | recommended for detection of G&alpha o of mouse, rat, human and bovine origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
