Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing Z discs and cytoplasmic staining of myocytes.Application
Western blot analysis of Desmin expression in non-transfected 293T: (A), mouse Desmin transfected 293T: (B) and SJRH30 (C) whole cell lysates.Application
Western blot analysis of Desmin expression in SJRH30 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoplasmic localization.| Product Name | Desmin Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 15-90 mapping near the N-terminus of desmin of human origin |
| Modification Notes | Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain å-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Desmin makes up attachments between the terminal Z-disc and membrane-associated proteins to form a force-transmitting system. Mutations in the gene encoding for Desmin are associated with adult-onset skeletal myopathy, sporadic disease and mild cardiac involvement. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of desmin of mouse, rat and human origin WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 15-90 mapping near the N-terminus of desmin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 15-90 mapping near the N-terminus of desmin of human origin |
| Entrez Gene | 1674 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing Z discs and cytoplasmic staining of myocytes.
Application
Western blot analysis of Desmin expression in non-transfected 293T: (A), mouse Desmin transfected 293T: (B) and SJRH30 (C) whole cell lysates.
Application
Western blot analysis of Desmin expression in SJRH30 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed Sol8 cells showing cytoplasmic localization.| Application Notes | recommended for detection of desmin of mouse, rat and human origin WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
