Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing membrane staining of myocytes.Application
Western blot analysis of T-cadherin expression in non-transfected: (A) and human T-cadherin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of T-cadherin expression in non-transfected: (A) and mouse T-cadherin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of T-cadherin expression in Jurkat (A) and PC-3 (B) whole cell lysates.| Product Name | T-cadherin Antibody (H-126) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 300-425 mapping within an internal region of T-cadherin of human origin |
| Modification Notes | Cadherins comprise a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogen-esis (1-4). Cadherins each contain a large extracellular domains characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity (5,6). The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ß-catenin, to regulate cadherin function (7). T-cadherin (for truncated-cadherin), also designated heart-cadherin or cadherin-13) expression levels have been shown to be reduced in human breast cancers and carcinoma cell lines. Evidence suggests that decreased levels of T-cadherin indicate a progression in breast malignancies (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of T-cadherin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 300-425 mapping within an internal region of T-cadherin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 300-425 mapping within an internal region of T-cadherin of human origin |
| Entrez Gene | 1012 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing membrane staining of myocytes.
Application
Western blot analysis of T-cadherin expression in non-transfected: (A) and human T-cadherin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of T-cadherin expression in non-transfected: (A) and mouse T-cadherin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of T-cadherin expression in Jurkat (A) and PC-3 (B) whole cell lysates.| Application Notes | recommended for detection of T-cadherin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
