Application
Western blot analysis of Epac2 expression in mouse brain (A) and mouse cerebellum (B) tissue extracts.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing membrane localization.Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.| Product Name | Epac2 Antibody (H-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | 3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac1 (cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the ras family GTPases Rap1 and Rap2 by promoting GTP binding in a cAMP-dependent manner (1,2). Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission (3). Human EPAC2 contains at least 31 exons and maps to chromosome 2q31 (4). The 4.4-kb Epac2 transcript is prominent in brain and adrenal gland (5,6). Within the brain, expression is strong in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus and cerebellum (5,6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Epac2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-220 mapping at the N-terminus of Epac2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-220 mapping at the N-terminus of Epac2 of human origin |
| Entrez Gene | 11069 |
|---|
Application
Western blot analysis of Epac2 expression in mouse brain (A) and mouse cerebellum (B) tissue extracts.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse brain tissue showing membrane localization.
Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Epac2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
