Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of 14-3-3 expression in HeLa (A), SK-N-SH (B) and PC-12 (C) whole cell lysates and mouse cerebellum tissue extract (D).Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and KNRK (C) whole cell lysates.Application
Note presence of IgG heavy chain using goat anti-rabbit IgG-HRP conventional secondary antibody: (A) as compared to the absence of heavy chain using ExactaCruz F: (B).Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and 14-3-3 β siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with 14-3-3 β (FL-246): .Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | pan-14-3-3 Antibody (FL-246) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-246 representing full length 14-3-3 β of human origin |
| Modification Notes | 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 ∫, ©, é, Ω, ñ, † and s. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of members of the 14-3-3 protein family of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-246 representing full length 14-3-3 &beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-246 representing full length 14-3-3 β of human origin |
| Entrez Gene | 7529 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of 14-3-3 expression in HeLa (A), SK-N-SH (B) and PC-12 (C) whole cell lysates and mouse cerebellum tissue extract (D).
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Western blot analysis of 14-3-3 ζ expression in non-transfected 293T: (A), human 14-3-3 ζ transfected 293T: (B) and KNRK (C) whole cell lysates.
Application
Note presence of IgG heavy chain using goat anti-rabbit IgG-HRP conventional secondary antibody: (A) as compared to the absence of heavy chain using ExactaCruz F: (B).
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and 14-3-3 β siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with 14-3-3 β (FL-246): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of members of the 14-3-3 protein family of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
