Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing cytoplasmic and membrane staining of squamous epithelial cells.Application
Western blot analysis of CKR-2A expression in Hep G2 (A), HEK293 (B), A-431 (C) and HL-60 (D) whole cell lysates.Application
Western blot analysis of CKR-2A expression in THP-1 whole cell lysate.| Product Name | CKR-2A Antibody (H-61) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | C-C or b chemokine family members are characterized by a pair of adjacent cysteine residues and serve as potent chemoattractants and activators of monocytes and T cells. C-C chemokine receptor family members include CKR-1, CKR-2A, CKR-2B, CKR-3, CKR-4, CKR-5, CKR-6, CKR-7, CKR-8, CKR-9, CKR-10 and the Duffy blood group antigen. Each of these receptors are G protein coupled, seven pass transmembrane domain proteins whose major physiological role is to function in the chemotaxis of T cells and phagocytic cells to areas of inflammation. However, this receptor family has also been shown to facilitate viral infection. CKR-2 (C-C chemokine receptor type 2) is a 374 amino acid multi-pass membrane protein that belongs to the C-C chemokine receptor family and is expressed as two isoforms, designated CKR-2A and CKR-2B. Both CKR-2 isoforms function as receptors for a variety of proteins, including MCP-1 and MCP-3, thereby influencing intracellular calcium levels and affecting signal transduction throughout the cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CKR-2A of human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 311-371 mapping near the C-terminus of CKR-2A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 311-371 mapping near the C-terminus of CKR-2A of human origin |
| Entrez Gene | 729230 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human uterine cervix tissue showing cytoplasmic and membrane staining of squamous epithelial cells.
Application
Western blot analysis of CKR-2A expression in Hep G2 (A), HEK293 (B), A-431 (C) and HL-60 (D) whole cell lysates.
Application
Western blot analysis of CKR-2A expression in THP-1 whole cell lysate.| Application Notes | recommended for detection of CKR-2A of human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
