Application
Western blot analysis of p67-phox expression in non-transfected: (A) and human p67-phox transfected: (B) 293T whole cell lysates.Application
Western blot analysis of p67-phox expression in non-transfected: (A) and mouse p67-phox transfected: (B) 293T whole cell lysates.Application
Western blot analysis of p67-phox expression in 293T whole cell lysate.| Product Name | p67-phox Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47phox and p67phox (1). The cytosolic proteins p47phox and p67phox, also designated neutrophil cytosol factor 1(NCF1) and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells (2,3). During activation of the NADPH oxidase, p47phox and p67phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex (4,5). Both p47phox and p67phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 domain of p67phox has been shown to interact with the proline rich domain of p47phox, suggesting that p47phox may faciliate the transport of p67phox to the membrane (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p67-phox of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of p67-phox of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of p67-phox of human origin |
| Entrez Gene | 4688 |
|---|
Application
Western blot analysis of p67-phox expression in non-transfected: (A) and human p67-phox transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of p67-phox expression in non-transfected: (A) and mouse p67-phox transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of p67-phox expression in 293T whole cell lysate.| Application Notes | recommended for detection of p67-phox of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
