Application
Western blot analysis of EF-2 expression in non-transfected 293T: (A), human EF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.Application
Western blot analysis of EF-2 expression in rat ovary tissue extract.| Product Name | EF-2 Antibody (H-118) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Two elongation factors (EF) EF-Tu and EF-2 participate in the elongation phase during protein biosynthesis on the ribosome and their functional cycles depend on GTP binding and its hydrolysis (1). EF-Tu (also designated mitochondrial precursor p43) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome where their low intrinsic GTPase activities are strongly stimulated (2). EF-Tu plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome (3). In addition, EF-Tu protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved (4). EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding (4,5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of EF-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 741-858 mapping at the C-terminus of EF-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 741-858 mapping at the C-terminus of EF-2 of human origin |
| Entrez Gene | 1938 |
|---|
Application
Western blot analysis of EF-2 expression in non-transfected 293T: (A), human EF-2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.
Application
Western blot analysis of EF-2 expression in rat ovary tissue extract.| Application Notes | recommended for detection of EF-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
