Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.Application
Western blot analysis of galectin-3 expression in non-transfected: (A) and human galectin-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of galectin-3 expression in non-transfected 293T: (A), human galectin-3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of galectin-3 expression in A-431 nuclear extract.Application
Western blot analysis of galectin-3 expression in HeLa (A) and MCF7 (B) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells (A) and immunoperoxidase staining of formalin fixed, paraffin-embedded mouse colon tissue (B) showing nuclear and cytoplasmic localization.| Product Name | galectin-3 Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Galectins are a family of soluble b-galactoside-binding animal lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) inter- actions and play a role in tumor progression, pre-mRNA splicing and apop-tosis. The galectin-3 protein, also known as Mac-2, hMac-2, GALBP, CBP35 or LGALS3, contains a single carbohydrate binding domain, which binds galactose-containing glycoconjugates. Galectin-3 is expressed in colonic and intestinal epithelium, inflammatory macrophages, papillary and follicular carcinomas, neoplastic astrocytes and some B and T lymphocytes. Upregulated expression of galectin-3 is involved in cancer progression and metastasis. Galectin-3 mediates the endocytosis of ∫1 Integrins in a lactose-dependent manner and is associated with thyroid malignancy and Crohn’s disease. It may also be used as a marker for diagnosing cases involving Hurthle cell adenomas and carcinomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-160 mapping at the N-terminus of galectin-3 of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-160 mapping at the N-terminus of galectin-3 of human origin |
| Entrez Gene | 3958 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing nuclear and cytoplasmic staining of urothelial cells.
Application
Western blot analysis of galectin-3 expression in non-transfected: (A) and human galectin-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of galectin-3 expression in non-transfected 293T: (A), human galectin-3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing nuclear and cytoplasmic staining of glandular cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of galectin-3 expression in A-431 nuclear extract.
Application
Western blot analysis of galectin-3 expression in HeLa (A) and MCF7 (B) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells (A) and immunoperoxidase staining of formalin fixed, paraffin-embedded mouse colon tissue (B) showing nuclear and cytoplasmic localization.| Application Notes | recommended for detection of galectin-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
