Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Conductin expression in DU 145 nuclear extract.| Product Name | Conductin Antibody (H-260) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 541-800 of Conductin of human origin |
| Modification Notes | β-catenin is a component of both the cadherin cell adhesion system and the Wnt signaling pathway. Wnt signaling increases the amount of β-catenin, by preventing its ubiquitination and degradation, allowing its direct interaction with transcription factors of the lymphoid enhancer factor-T cell factor family and modulation of gene expression. Axin is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3β. APC, which is phosphorylated by GSK-3β, induces degradation of β-catenin, thus inhibiting Wnt signal transduction. Conductin is 45% identical to axin and appears to play a similar role to axin in the Wnt signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Conductin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 541-800 of Conductin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 541-800 of Conductin of human origin |
| Entrez Gene | 8313 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Conductin expression in DU 145 nuclear extract.| Application Notes | recommended for detection of Conductin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
