Application
Western blot analysis of SV40 T Ag expression in XPro-12 (A) and GM637 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse colon tissue from transgenic mice over-expressing SV40 T-antigen from an intestine-specific promoter construct. Kindly provided by J. Gum, S. Crawley and S. Yang of the VA Medical Center, San Francisco.| Product Name | SV40 T Ag Antibody (v-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Simian virus SV40 has provided an important model for studies of cellular mechanisms involved in a malignant transformation. The major SV40 translational products include the large T antigen and the small T antigen, both of which are encoded by the early region of the SV40 viral ge-nome. The large T antigen complexes with the p53 suppressor gene, resulting in its functional inactivation, thus promoting cell transformation. In addition, SV40 large T antigen binds DNA polymerase and the transcription factor AP-2. It also forms complexes with a second tumor supressor gene-encoded protein, Rb 105. Binding of SV40 T antigen is specific for the “pocket” domain of Rb p105, which is also the binding site for the E2F cellular transcription factor. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SV40 Large T antigen (94 kDa) and Small T antigen (21 kDa) by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4-30 of the SV40 Large T antigen |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4-30 of the SV40 Large T antigen |
Application
Western blot analysis of SV40 T Ag expression in XPro-12 (A) and GM637 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse colon tissue from transgenic mice over-expressing SV40 T-antigen from an intestine-specific promoter construct. Kindly provided by J. Gum, S. Crawley and S. Yang of the VA Medical Center, San Francisco.| Application Notes | recommended for detection of SV40 Large T antigen (94 kDa) and Small T antigen (21 kDa) by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
