Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomerulus and tubules.Application
Western blot analysis of SPARC expression in non-transfected: (A) and human SPARC transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SPARC expression in A549 whole cell lysate.Application
Western blot analysis of SPARC expression in non-transfected: (A) and human SPARC transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SPARC expression in A-375 (A) and U-2 OS (B) whole cell lysates.| Product Name | SPARC Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SPARC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-90 mapping at the N-terminus of SPARC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-90 mapping at the N-terminus of SPARC of human origin |
| Entrez Gene | 6678 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomerulus and tubules.
Application
Western blot analysis of SPARC expression in non-transfected: (A) and human SPARC transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SPARC expression in A549 whole cell lysate.
Application
Western blot analysis of SPARC expression in non-transfected: (A) and human SPARC transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SPARC expression in A-375 (A) and U-2 OS (B) whole cell lysates.| Application Notes | recommended for detection of SPARC of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
