Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pre-menopausal uterus tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of FTβ expression in non-transfected 293T: (A), human FTβ transfected 293T: (B), A-431 (C) and JAR (D) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human endometrium tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.Application
Western blot analysis of FTβ expression in A-431 (A) and 3611-RF (B) whole cell lysates.| Product Name | FTβ Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical å and ∫ subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins (1). The natural substrates contain the Cys-A-A-Xaa recognition sequence (2), where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine (3,4). The purified farnesyl transferase is an a-b heterodimer (1,5). The ∫ subunit, which is known as FT∫, CAAX farnesyltransferase subunit ∫, or Ras proteins prenyltransferase subunit ∫, is a 437 amino acid protein that contains five PFTB repeats and binds the peptide substrate (6). The å subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FT&beta of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 138-437 mapping at the C-terminus of the 46 kDa &beta subunit of farnesyl transferase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 138-437 mapping at the C-terminus of the 46 kDa β subunit of farnesyl transferase of human origin |
| Entrez Gene | 2342 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pre-menopausal uterus tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of FTβ expression in non-transfected 293T: (A), human FTβ transfected 293T: (B), A-431 (C) and JAR (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human endometrium tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing cytoplasmic localization.
Application
Western blot analysis of FTβ expression in A-431 (A) and 3611-RF (B) whole cell lysates.| Application Notes | recommended for detection of FT&beta of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
