Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of OST48 expression in MDCK whole cell lysate.| Product Name | OST48 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Membrane proteins of the endoplasmic reticulum (ER) may be localized by mechanisms that involve retention, retrieval, or a combination of both. ER localization information has been found in cytoplasmic, transmembrane, or luminal domains. Specific retrieval mechanisms have been identified for luminal ER proteins, which contain a KDEL domain, and for type I transmembrane proteins carrying a dilysine motif. The mammalian oligosaccharyltransferase (OST) is a protein complex that is composed of four rough ER-specific, type I transmembrane proteins: ribophorins I and II (RI and RII), OST48, and DAD1 (also designated defender against apoptotic death). The ribophorins are integral membrane glycoproteins that localize exclusively to the rough endoplasmic reticulum. There is affinity between the cytoplasmically located N-terminal region of the DAD1 and the short cytoplasmic tail of OST48 to place DAD1 firmly into the OST complex. The OST affects the cotranslational N-glycosylation of newly synthesized polypeptides. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of OST48 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 157-456 mapping to the C-terminus of OST48 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 157-456 mapping to the C-terminus of OST48 of human origin |
| Entrez Gene | 1650 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of OST48 expression in MDCK whole cell lysate.| Application Notes | recommended for detection of OST48 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
