Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic and membrane staining of squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.Application
Western blot analysis of STEAP expression in LNCaP whole cell lysate.| Product Name | STEAP Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Six-transmembrane epithelial antigen of the prostate (STEAP) is structurally similar to a channel or transport protein (1,2). STEAP protein contains six potential membrane-spanning regions with hydrophilic amino- and carboxyl-terminal domains (1,2). STEAP protein is present in human prostate tissue with elevated levels in cancer cell lines, including prostate, bladder, colon, ovarian, and Ewing sarcoma (1–3). Cell-cell junctions of the secretory epithelium show concentrated levels of STEAP protein (2). Mouse STEAP is 80% homologous to human STEAP at both the nucleotide and amino acid levels (4). The human STEAP gene maps to chromosome 7q21 and encodes a 339 amino acid protein (1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of STEAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-105 mapping at the N-terminus of STEAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-105 mapping at the N-terminus of STEAP of human origin |
| Entrez Gene | 26872 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing cytoplasmic and membrane staining of squamous epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.
Application
Western blot analysis of STEAP expression in LNCaP whole cell lysate.| Application Notes | recommended for detection of STEAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
