Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing granular cytoplasmic staining of cells in tubules.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization.Application
Western blot analysis of HSP 60 expression in mouse placenta tissue extract (A) and HeLa whole cell lysate (B).| Product Name | HSP 60 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes, and the regulation of protein folding. HSPs (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins, and the HSP 110 family. The constitutively expressed mitochondrial protein HSP 60 shares the ability to recognize and stabilize proteins during folding, assembly and disassembly with other HSP family members. The mitochondrial and cytosolic localization of HSP 60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker for ©/∂ T cell recognition. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HSP 60 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 274-573 mapping at the C-terminus of HSP 60 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 274-573 mapping at the C-terminus of HSP 60 of human origin |
| Entrez Gene | 3329 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing granular cytoplasmic staining of cells in tubules.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing mitochondrial localization.
Application
Western blot analysis of HSP 60 expression in mouse placenta tissue extract (A) and HeLa whole cell lysate (B).| Application Notes | recommended for detection of HSP 60 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
