Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Crk-L expression in non-transfected: (A) and human Crk-L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Crk-L expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of Crk-L expression in non-transfected: (A) and human Crk-L transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Crk-L expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | Crk-L Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SH2 and SH3 (Src homology) domains were originally identified as critical functional domains within non-receptor proteins with tyrosine kinase activity. A subset of these proteins appears to exist predominately of SH2/SH3 domains in the absence of detectable catalytic domains. One of the first members of the family to be identified, Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Crk-L p36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Crk-L of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Crk-L of human origin |
| Entrez Gene | 1399 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Crk-L expression in non-transfected: (A) and human Crk-L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Crk-L expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of Crk-L expression in non-transfected: (A) and human Crk-L transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Crk-L expression in A-431 (A) and NIH/3T3 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Crk-L p36 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
