Application
Immunofluorescence staining of methanol-fixed EOC 20 cells showing cytoplasmic localization.| Product Name | A cyclase IV Antibody (R-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 631-800 mapping near the C-terminus of adenylyl cyclase IV of rat origin |
| Modification Notes | Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules (1,2). Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins (3). The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Ga s) for GTP, the dissociation of GTP-Ga s from Gbg and Ga s-mediated activation of adenylyl cyclase (3,4). Adenylyl cyclase IV (AC IV) and IX mRNA are expressed in all kidney nephron segments (5). AC IV exhibits moderate staining in type II and type IV fibrocytes in rat cochlea and immunoreactivity is also obersed in type I fibrocytes (6). AC IV is expressed in myenteric ganglia and is variably expressed in glia of the duodenum, jejunum and ileum (7). Activation of the D2 dopaminergic and m4 muscarine receptors inhibits the activity of adenylyl cyclase isozymes I, V, VI and VIII, whereas type II, IV and VII are stimulated and type III is not affected (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of adenylyl cyclase IV of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 631-800 mapping near the C-terminus of adenylyl cyclase IV of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 631-800 mapping near the C-terminus of adenylyl cyclase IV of rat origin |
| Entrez Gene | 196883 |
|---|
Application
Immunofluorescence staining of methanol-fixed EOC 20 cells showing cytoplasmic localization.| Application Notes | recommended for detection of adenylyl cyclase IV of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
