Application
Western blot analysis of TLR4 expression in THP-1 whole cell lysate.Application
Western blot analysis of TLR4 expression in HEL 92.1.7 (A) and HL-60 (B) whole cell lysates.WB
PLPE and Met restored hepatic insulin signaling through JNK inhibition and NF-κB activation. Parameters shown: hepatic protein expression of TLR4 (A), P-JNK/T-JNK (B), IκBα (C), and P-IRS1/T-IRS1 (D).WB
Inflammatory response was suppressed by Lactobacillus treatment. Protein expression levels in colonic tissue of TLR4 and IκBα (A, B). Serum levels of pro-inflammatory cytokines TNF-α and IL-6 (C, D). Statistical notations as in Figure 1.| Product Name | TLR4 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Six human homologs of the Drosophila Toll receptor were initially identified based on their sequence similarities and designated toll-like receptors (TLR). Toll receptors are involved in mediating dorsoventral polarization in the developing Drosophila embryo and also participate in the host immunity. The TLR family of proteins are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transduction. TLR1, as well as the other TLR family members, are type I transmembrane receptors that characteristically contain an extracellular domain consisting of several leucine-rich regions along with a single cytoplasmic Toll/IL-1R-like domain. TLR2 and TLR4 are activated in response to lipopolysacchride (LPS) stimulation, which results in the activation and translocation of NFkB and suggests that these receptors are involved in mediating inflammatory responses. Expression of TLR receptors is highest in peripheral blood leukocytes, macro-phages, and monocytes. TLR6 is highly homologous to TLR1, sharing greater than 65% sequence identity, and, like other members of TLR family, it induces NFkB signaling upon activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 242-321 mapping to an internal region of TLR4 (Toll-like receptor 4) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 242-321 mapping to an internal region of TLR4 (Toll-like receptor 4) of human origin |
| Entrez Gene | 7099 |
|---|
Application
Western blot analysis of TLR4 expression in THP-1 whole cell lysate.
Application
Western blot analysis of TLR4 expression in HEL 92.1.7 (A) and HL-60 (B) whole cell lysates.
WB
PLPE and Met restored hepatic insulin signaling through JNK inhibition and NF-κB activation. Parameters shown: hepatic protein expression of TLR4 (A), P-JNK/T-JNK (B), IκBα (C), and P-IRS1/T-IRS1 (D).
WB
Inflammatory response was suppressed by Lactobacillus treatment. Protein expression levels in colonic tissue of TLR4 and IκBα (A, B). Serum levels of pro-inflammatory cytokines TNF-α and IL-6 (C, D). Statistical notations as in Figure 1.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
