Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of Jagged1 expression in MIA PaCa-2 whole cell lysate.Application
Western blot analysis of Jagged1 expression in HeLa (A) and Hs 67 (B) whole cell lysates.Application
Western blot analysis of human recombinant Jagged1 fusion protein.| Product Name | Jagged1 Antibody (H-66) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 207-272 mapping near the N-terminus of Jagged1 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged is a membrane protein and can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis. Jagged is expressed in adult and fetal tissues, and expression is upregulated in cervical squamous cell carcinoma. Familial Tetralogy of Fallot, the most common form of complex congenital heart disease, is caused by a mutation in the Jagged1 gene. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 207-272 mapping near the N-terminus of Jagged1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 207-272 mapping near the N-terminus of Jagged1 of human origin |
| Entrez Gene | 182 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of Jagged1 expression in MIA PaCa-2 whole cell lysate.
Application
Western blot analysis of Jagged1 expression in HeLa (A) and Hs 67 (B) whole cell lysates.
Application
Western blot analysis of human recombinant Jagged1 fusion protein.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
