Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharinx tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of A cyclase I expression in Y79 (A), ARPE-19 (B) and IMR-32 (C) whole cell lysates.| Product Name | A cyclase I Antibody (V-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of A cyclase I of human origin |
| Modification Notes | Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules (1,2). Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Ga s) for GTP, the dissociation of GTP-Gå s from G∫© and Ga s-mediated activation of adenylyl cyclase (3,4). A cyclase I, also known as AC1 or ADCY1, is a 1,119 amino acid multi-pass membrane protein expressed in the brain, retina and adrenal medulla. A cyclase I binds two magnesium ions per subunit and may be involved in regulatory processes in the central nervous system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of adenylyl cyclase I of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of A cyclase I of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of A cyclase I of human origin |
| Entrez Gene | 107 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharinx tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of A cyclase I expression in Y79 (A), ARPE-19 (B) and IMR-32 (C) whole cell lysates.| Application Notes | recommended for detection of adenylyl cyclase I of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
