Application
Western blot analysis of CaMKKα expression in non-transfected 293T: (A), human CaMKKα transfected 293T: (B) and PC-12 (C) whole cell lysates.Application
Western blot analysis of CaMKKα expression in non-transfected: (A) and human CaMKKα transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining in cortical cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining in cortical cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.Application
Western blot analysis of CaMKKα expression in HeLa (A) and PC-12 (B) whole cell lysates and rat brain (C) extract.| Product Name | CaMKKα Antibody (R-73) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-73 mapping at the N-terminus of CaMKKα of rat origin |
| Modification Notes | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed Serine/Threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated å, ∫, © and ∂, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CaMKKα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-73 mapping at the N-terminus of CaMKK&alpha of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-73 mapping at the N-terminus of CaMKKα of rat origin |
| Entrez Gene | 84254 |
|---|
Application
Western blot analysis of CaMKKα expression in non-transfected 293T: (A), human CaMKKα transfected 293T: (B) and PC-12 (C) whole cell lysates.
Application
Western blot analysis of CaMKKα expression in non-transfected: (A) and human CaMKKα transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of cortical cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining in cortical cells (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining in cortical cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse liver frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Application
Western blot analysis of CaMKKα expression in HeLa (A) and PC-12 (B) whole cell lysates and rat brain (C) extract.| Application Notes | recommended for detection of CaMKKα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
