Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of GRB2 expression in Jurkat whole cell lysate.Application
Intracellular FCM analysis of fixed and permeabilized PC-12 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of GRB2 expression in Jurkat immunoprecipitated with GRB2 (1-68): and detected with GRB2 (G-23): .Application
Western blot analysis of mouse recombinant GRB2 fusion protein.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing specific staining of membrane ruffles (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing membrane staining of mucosal epithelia (B).Application
Western blot analysis of GRB2 expression in PC-12 (A) and KNRK (B) whole cell lysates.| Product Name | GRB2 Antibody (C-23) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of GRB2 of human origin |
| Modification Notes | The superfamily of GTP binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. A family of guanine nucleotide releasing factors (GRFs) activate Ras in mammalian cells and growth factor receptor-bound protein 2 (GRB2), an adaptor protein (also referred to as Sem 5) that appears to mediate the interaction of GRFs with activated receptor molecules. GRB2 forms a complex with activated EGFR (epidermal growth factor receptor) and the Ras-specific guanine nucleotide exchange factor SOS1, and, together, they regulate the growth factor-induced activation of Ras. GRB2 exhibits both structural and functional homology to the C. elegans protein sem-5. GRB2 is necessary during embryogenesis for the differentiation of endodermal cells and formation of the epiblast. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of GRB2 p25 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of GRB2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of GRB2 of human origin |
| Entrez Gene | 2885 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of GRB2 expression in Jurkat whole cell lysate.
Application
Intracellular FCM analysis of fixed and permeabilized PC-12 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of GRB2 expression in Jurkat immunoprecipitated with GRB2 (1-68): and detected with GRB2 (G-23): .
Application
Western blot analysis of mouse recombinant GRB2 fusion protein.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing specific staining of membrane ruffles (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing membrane staining of mucosal epithelia (B).
Application
Western blot analysis of GRB2 expression in PC-12 (A) and KNRK (B) whole cell lysates.| Application Notes | recommended for detection of GRB2 p25 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
