Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and cytoplasmic staining of glandular cells.Application
Western blot analysis of Cyr61 expression in FHs 173We whole cell lysate.Application
Western blot analysis of Cyr61 expression in non-transfected: (A) and human Cyr61 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cyr61 expression in non-transfected: (A) and human Cyr61 transfected: (B) 293 whole cell lysates.| Product Name | Cyr61 Antibody (H-78) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cyr61 is a secreted heparin binding protein, encoded by a growth factor-inducible immediate-early gene, that associates with the extracellular matrix and connective tissue. Cyr61 is a member of a distinct family of angiogenic and vasculogenic regulators designated CCN proteins, which includes connective tissue growth factor (CTGF) and the mouse Cyr61 homolog, Fisp12. As an angiogenic inducer, Cyr61 binds to the cell surface receptor Integrin aV/b3, where it then stimulates cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Expression of Cyr61 is elevated during vessel growth, wound healing and chondrocyte differentiation. Cyr61 is also detected in a wide variety of tumors as it induces tumor growth and functions as a marker of tumor progression. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cyr61 of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA also reactive with additional species, including equine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 163-240 of Cyr61 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 163-240 of Cyr61 of human origin |
| Entrez Gene | 3491 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and cytoplasmic staining of glandular cells.
Application
Western blot analysis of Cyr61 expression in FHs 173We whole cell lysate.
Application
Western blot analysis of Cyr61 expression in non-transfected: (A) and human Cyr61 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cyr61 expression in non-transfected: (A) and human Cyr61 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of Cyr61 of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA also reactive with additional species, including equine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
