Application
Western blot analysis of Ska2 expression in mouse brain tissue extract.Application
Western blot analysis of Ska2 expression in NIH/3T3 (A), 3T3-L1 (B), A549 (C) and Jurkat (D) whole cell lysates and MCF7 (E) and HeLa (F) nuclear extracts.| Product Name | Ska2 Antibody (S-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Ska2 (spindle and kinetochore associated complex subunit 2), also known as FAM33A, is a 121 amino acid component of the Ska1 complex, a microtubule-binding subcomplex of the outer kinetochore that is critical for proper chromosome segregation. The Ska1 complex is a component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. Localized to the outer kinetochore and spindle microtubules during cell proliferation, Ska2 is essential for spindle checkpoint silencing and exit from mitosis. Downregulation of Ska2 leads to delayed recruitment of MAD2, a component of the mitotic spindle checkpoint, to several kinetochores resulting in occasional loss of individual chromosomes from the metaphase plate. Ska2 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ska2 and Ska2L of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Ska1 family member | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of Ska2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of Ska2 of human origin |
| Entrez Gene | 348235 |
|---|
Application
Western blot analysis of Ska2 expression in mouse brain tissue extract.
Application
Western blot analysis of Ska2 expression in NIH/3T3 (A), 3T3-L1 (B), A549 (C) and Jurkat (D) whole cell lysates and MCF7 (E) and HeLa (F) nuclear extracts.| Application Notes | recommended for detection of Ska2 and Ska2L of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with Ska1 family member |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
