Application
Western blot analysis of MEK kinase-2 expression in non-transfected: (A) and mouse MEK kinase-2 transfected: (B) 293T whole cell lysates.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with MEK kinase-2 (N-19), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic staining.Application
Western blot analysis of MEK kinase-2 expression in MCF7 (A), Jurkat (B), BJAB (C), Ramos (D) and K-562 (E) whole cell lysates.| Product Name | MEK kinase-2 Antibody (N-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of MEK kinase-2 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of MEK kinase-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of MEK kinase-2 of human origin |
| Entrez Gene | 10746 |
|---|
Application
Western blot analysis of MEK kinase-2 expression in non-transfected: (A) and mouse MEK kinase-2 transfected: (B) 293T whole cell lysates.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with MEK kinase-2 (N-19), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic staining.
Application
Western blot analysis of MEK kinase-2 expression in MCF7 (A), Jurkat (B), BJAB (C), Ramos (D) and K-562 (E) whole cell lysates.| Application Notes | recommended for detection of MEK kinase-2 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
