Application
Western blot analysis of mrnp41 expression in non-transfected: (A) and human mrnp41 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of mrnp41 expression in non-transfected 293T: (A), human mrnp41 transfected 293T: (B), HeLa (C), MCF7 (D) and NIH/3T3 (E) whole cell lysates and HeLa nuclear extract (F).Application
mrnp41 (D-18): Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | mrnp41 Antibody (D-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mrnp41 (mRNA-binding protein, 41-KD), also known as Rae1 protein homolog and mRNA export factor, is a 368 amino acid protein that binds mRNA and is involved in nucleocytoplasmic transport. Though characterized in both the nucleus and cytoplasm, mrnp41 is primarily localized to the nuclear pore complex in the nuclear envelope. Mutations in mrnp41 may result in the accumulation of poly(A)-containing mRNA in the nucleus, further supporting the role of mrnp41 as a primary nuclear exporter of mRNA. Along with Nup98, mrnp41 has been shown to regulate E-cadherin, an activating subunit of the anaphase-promoting complex complex, which results in the prevention of securin degradation, therefore suggesting that mrnp41 may play a potential role in maintaining euploidy. Also, during mitosis, both mrnp41 and NuMA have been shown to be required for bipolar spindle formation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of mrnp41 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of mrnp41 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of mrnp41 of human origin |
| Entrez Gene | 8480 |
|---|
Application
Western blot analysis of mrnp41 expression in non-transfected: (A) and human mrnp41 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of mrnp41 expression in non-transfected 293T: (A), human mrnp41 transfected 293T: (B), HeLa (C), MCF7 (D) and NIH/3T3 (E) whole cell lysates and HeLa nuclear extract (F).
Application
mrnp41 (D-18): Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | recommended for detection of mrnp41 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
