Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.Application
Western blot analysis of GD3 Synthase expression in non-transfected: (A) and mouse GD3 Synthase transfected: (B) 293T whole cell lysates.| Product Name | GD3 Synthase Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | GD3 Synthase (GD3S, SIAT8, ST8SiaI, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1) is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. GD3 Synthase is found in the Golgi apparatus and is a member of glycosyltransferase family 29. GD3 synthase can down-regulate MMP-9 promoter activity in response to TNF-alpha by association with NF-kappaB and activation protein-1 (AP-1) sites in the MMP-9 promoter. GD3 Synthase has an apoptotic effect on ECV304 cells through downregulation of Bcl-2 expression via dephosphorylation of AKT and CREB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of GD3 Synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 65-140 mapping near the N-terminus of GD3 Synthase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 65-140 mapping near the N-terminus of GD3 Synthase of human origin |
| Entrez Gene | 6489 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.
Application
Western blot analysis of GD3 Synthase expression in non-transfected: (A) and mouse GD3 Synthase transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of GD3 Synthase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
