Application
Western blot analysis of PKC δ expression in NIH/3T3 whole cell lysate.Application
Antibodies tested include PKC δ (C-17): (A,B) and p-PKC δ (Ser 643): (C,D).Application
Western blot analysis of PKC δ expression in A-431 whole cell lysate.Application
Antibodies tested include PKC δ (C-17): (A) and PKC δ (C-20): (B-E).Application
Immunofluorescence staining of methanol/acetone fixed rat embryo fibroblasts using fluorescein-labeled goat anti-rabbit IgG secondary antibody.| Product Name | PKC δ Antibody (C-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PKC δ of rat origin |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (å, ∫I, ∫II, ©, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAChIP |
| recommended for detection of PKC δ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKC &delta of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKC δ of rat origin |
| Entrez Gene | 5580 |
|---|
Application
Western blot analysis of PKC δ expression in NIH/3T3 whole cell lysate.
Application
Antibodies tested include PKC δ (C-17): (A,B) and p-PKC δ (Ser 643): (C,D).
Application
Western blot analysis of PKC δ expression in A-431 whole cell lysate.
Application
Antibodies tested include PKC δ (C-17): (A) and PKC δ (C-20): (B-E).
Application
Immunofluorescence staining of methanol/acetone fixed rat embryo fibroblasts using fluorescein-labeled goat anti-rabbit IgG secondary antibody.| Application Notes | recommended for detection of PKC δ of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
