Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular and lymphoid cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of HePTP expression in Jurkat (A), Jurkat + IL-2 (B) and Ramos (C) whole cell lysates and mouse thymus (D) tissue extract.Application
Western blot analysis of HePTP expression in IL-2-treated Jurkat whole cell lysate.| Product Name | HePTP Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 46-125 of HePTP of human origin |
| Modification Notes | Hematopoietic protein tyrosine phosphatase (HePTP) belongs to a subgroup of PTPases with two other members, STEP and PCPTP1, all of which consist of a single C-terminal PTPase domain that is preceded by a noncatalytic N-terminal domain. Unlike STEP and PCPTP1, which are expressed primarily in the central nervous system, HePTP is expressed in thymus, spleen and in leukemic cell lines, including Jurkat T leukemia cells. The gene encoding HePTP was originally cloned from human T lymphocytes, and it maps to chromosome 1q32.1, a site frequently mutated in preleukemic myeloproliferative disease. The locus of the gene suggests a role for HePTP in cell proliferation and differentiation. The HePTP gene is transcriptionally activated in T cells treated with Interleukin 6. HePTP mRNA levels increase several-fold in normal mouse lymphocytes upon stimulation with phytohemagglutinin, lipopolysaccharide, concanavalin A and anti-CD3. Overexpression of HePTP reduces T cell receptor (TCR)-induced activation of ERK 2, and interferes with PMA and growth factor-induced MAPK activation in myeloid cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HePTP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 46-125 of HePTP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 46-125 of HePTP of human origin |
| Entrez Gene | 5778 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular and lymphoid cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of HePTP expression in Jurkat (A), Jurkat + IL-2 (B) and Ramos (C) whole cell lysates and mouse thymus (D) tissue extract.
Application
Western blot analysis of HePTP expression in IL-2-treated Jurkat whole cell lysate.| Application Notes | recommended for detection of HePTP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
