Application
Western blot analysis of EHMT1 expression in cnon-transfected control (A) and EHMT1 siRNA transfected (B) HeLa cell lysates. Kindly provided by The Dr. Michael Rosenfeld Laboratory, University of California San Diego.Application
Western blot analysis of EHMT1 expression in non-transfected (A) and human EHMT1 transfected (B) 293 whole cell lysates.| Product Name | EHMT1 Antibody (T-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of EHMT1 of human origin by WB, IP and ELISA |
| Modification Notes | EHMT1 (also known as euchromatic histone-lysine N-methyltransferase 1) is a widely expressed histone methyltransferase. EHMT1 belongs to the histone-lysine methyltransferase family and contains eight ANK repeats, one pre-SET domain and one SET domain. It acts to methylate Lys 9 of Histone H3, which represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. During G0 phase, EHMT1 is found as part of the E2F6.com-1 complex and probably contributes to silencing of Myc- and E2F-responsive genes, suggesting a role in G0/G1 transition in the cell cycle. EHMT1 defects are the cause of chromosome 9q subtelomeric deletion syndrome. Common indicators of this syndrome are severe mental retardation, hypotonia, brachy(micro)cephaly, epileptic seizures, synophrys, prognathism, macroglossia and conotruncal heart defects. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of EHMT1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of EHMT1 of human origin by WB, IP and ELISA |
| Entrez Gene | 79813 |
|---|
Application
Western blot analysis of EHMT1 expression in cnon-transfected control (A) and EHMT1 siRNA transfected (B) HeLa cell lysates. Kindly provided by The Dr. Michael Rosenfeld Laboratory, University of California San Diego.
Application
Western blot analysis of EHMT1 expression in non-transfected (A) and human EHMT1 transfected (B) 293 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
