Application
Western blot analysis of GABAA Rγ2 phosphorylation in rat prefrontal cortex tissue extract. Blots were probed with p-GABAA Rγ2 (Ser 365): (A) and p-GABAA Rγ2 (Ser 365): preincubated with its cognate phosphorylated peptide (B).| Product Name | p-GABAA Rγ2 Antibody (Ser 365) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 365 phosphorylated GABAA Rγ2 of mouse and rat origin and correspondingly Ser 366 phosphorylated GABAA Rγ2 of human origin of mouse, rat and human origin by WB and IP; also reactive with additional species, inclu |
| Modification | Phosphoralated |
| Modification Notes | GAD-65 and GAD-67, glutamate decarboxylases, function to catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system, GABA functions as the main inhibitory transmitter by increasing a Cl- (chloride) conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors, as well as a third class of receptors called GABAC. The © subunit of GABAA receptors are important for benzodiazepine binding and modulation of GABA-mediated Cl- current. GABAA R©2 is a 467 amino acid mulit-pass membrane protein localized to the postsynaptic cell membrane. Present as a pentamer with other GABAA receptor chains (å, ∫, ©, ∂ and r), the GABAA ligand-gated Cl- channels selectively complex with D5DR to enable mutual inhibitory functional interactions between the two receptor systems. Defects in the gene encoding GABAA R©2 have been found to be the cause of childhood absence epilepsy type 2, familial febrile convulsions type 8, generalized epilepsy with febrile seizures plus type 3 and severe myoclonic epilepsy in infancy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 365 phosphorylated GABAA R&gamma 2 of mouse and rat origin and correspondingly Ser 366 phosphorylated GABAA R&gamma 2 of human origin of mouse rat and human origin by WB and IP also reactive with additional species inclu |
|---|---|
| Tissue Specificity | recommended for detection of Ser 365 phosphorylated GABAA Rγ2 of mouse and rat origin and correspondingly Ser 366 phosphorylated GABAA Rγ2 of human origin of mouse, rat and human origin by WB and IP; also reactive with additional species, including bovine and canine |
| Entrez Gene | 2566 |
|---|
Application
Western blot analysis of GABAA Rγ2 phosphorylation in rat prefrontal cortex tissue extract. Blots were probed with p-GABAA Rγ2 (Ser 365): (A) and p-GABAA Rγ2 (Ser 365): preincubated with its cognate phosphorylated peptide (B).| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
