Application
Western blot analysis of phosphorylated c-Jun expression in untreated (A) and UV-treated (B) HeLa whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Product Name | p-c-Jun Antibody (Thr 239) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 239 of c-Jun of human origin |
| Modification | Phosphoralated |
| Modification Notes | Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are found to be associated with a number of transcriptional complexes (1,2). The c-Jun protein is a major component of the transcription factor AP-1 (3,4), originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE) (3,4). The Jun proteins form homo- and heterodimers which bind the TRE, but the Fos proteins are active only as heterodimers with any of the Jun proteins (5,6). Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain (7). An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is deactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA) (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 239 phosphorylated c-Jun of human origin and correspondingly phosphorylated Thr 242 of mouse and rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 239 of c-Jun of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 239 of c-Jun of human origin |
| Entrez Gene | 3725 |
|---|
Application
Western blot analysis of phosphorylated c-Jun expression in untreated (A) and UV-treated (B) HeLa whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Application Notes | recommended for detection of Thr 239 phosphorylated c-Jun of human origin and correspondingly phosphorylated Thr 242 of mouse and rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
