Application
Western blot analysis of PKC η expression in non-transfected 293T: (A), human PKC η transfected 293T: (B) and WI 38 (C) whole cell lysates.Application
Western blot analysis of PKC η expression in non-transfected: (A) and human PKC η transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol/acetone fixed rat embryo fibroblasts both with p-Tyr (PY20): (rhodamine) and nPKC η (C-15): (fluorescein).Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).Application
Immunofluorescence staining of methanol/acetone fixed rat embryo fibroblasts with both PKC η (C-15): (fluorescein) and p-Tyr (PY20): (rhodamine).Application
Western blot analysis of PKC η expression in rat (A) and mouse (B) lung tissue extracts.| Product Name | PKC η Antibody (C-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PKC η of mouse origin |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (å, ∫I, ∫II, ©, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PKC η of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKC &eta of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKC η of mouse origin |
| Entrez Gene | 5583 |
|---|
Application
Western blot analysis of PKC η expression in non-transfected 293T: (A), human PKC η transfected 293T: (B) and WI 38 (C) whole cell lysates.
Application
Western blot analysis of PKC η expression in non-transfected: (A) and human PKC η transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol/acetone fixed rat embryo fibroblasts both with p-Tyr (PY20): (rhodamine) and nPKC η (C-15): (fluorescein).
Application
PKC antibodies used include PKC α (C-20), PKC βI (C-16), PKC βII (C-18), PKC γ (C-19), PKC ε (C-15), PKC ζ (C-20) and PKC η (C-15).
Application
Immunofluorescence staining of methanol/acetone fixed rat embryo fibroblasts with both PKC η (C-15): (fluorescein) and p-Tyr (PY20): (rhodamine).
Application
Western blot analysis of PKC η expression in rat (A) and mouse (B) lung tissue extracts.| Application Notes | recommended for detection of PKC η of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
